Seahorse XFe96 Analyzer

Seahorse XFe96 Analyzers measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.

For Research Use Only. Not for use in diagnostic procedures.



  • Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling. Wave software controls the instrument and performs rate measurements on the fly for same day results.
  • Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
  • Flexible Assay Design- the 96-well plate format accommodates many conditions in a single run and is best for dose-response studies and compound screening.
  • High Sensitivity- analyze as few as 5000 cells per well in the custom 96-well plate. Cell number requirements vary by cell type; consult the Cell Reference Database for details.
  • Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).
  • Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test.
  • Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test.
  • Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay.
  • Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test.
  • Analyze 3D samples such as spheroids and islets with the Seahorse XFe96 Spheroid Plate.
  • Easily create assay protocols and analyze data with Seahorse Wave software.


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