The Single-Cell Secretome solution enables the discovery of better biomarkers and accelerated development through functional immune landscaping of each immune cell, allowing for complete single-cell functional characterization. Detect rare subsets of “super cells” to reveal functional biological drivers of persistence, potency, durability and more.

32+ Cytokine Analysis

Multiplexed 32+ cytokine analysis at single-cell resolution

Functional Phenotyping

Functional Phenotyping targeting 500-1500 single cells per chip

Fully Automated

Fully automated, quick, and cost-efficient workflow

Human Adaptive Immune

Granzyme B, IFN-γ, MIP-1α, Perforin, TNF-α, TNF-β, GM-CSF, IL-2, IL-5, IL-7, IL-8, IL-9, IL-12,
IL-15, IL-21, CCL11, IP-10, MIP-1β, RANTES, IL-4, IL-10, IL-13, IL-22, TGFβ1, sCD137, sCD40L,
IL-1β, IL-6, IL-17A, IL-17F, MCP-1, MCP-4

Non-Human Primate Adaptive Immune

TNF-α, MCP-1, IL-2, IL-4, MIP-1β, IL-6, IL-8, IL-1β, RANTES, IFN-g, IP-10, MIP-1α, MIF, GM-CSF

Mouse Adaptive Immune

Granzyme B, IFN-γ, MIP-1α, TNF-α, GM-CSF, IL-2, IL-5, IL-7, IL-12p70, IL-15, IL-18, IL-21,
sCD137, CCL11, CXCL1, CXCL13, IP-10, RANTES, Fas, IL-4, IL-10, IL-13, IL-27, TGFβ1, IL-6,
IL-17A, MCP-1, IL-1β

Human Innate Immune

IFN-γ, MIP-1α, TNF-α, TNF-β, GM-CSF, IL-8, IL-9, IL-15, IL-18, TGF-α, IL-5, CCL11, IP-10, MIP-1β,
RANTES, BCA-1, IL-10, IL-13, IL-22, sCD40L, IL-1β, IL-6, IL-12-p40, IL-12, IL-17A, IL-17F, MCP-1,

Human Inflammation

GM-CSF, IFN-γ, IL-2, IL-12, TNF-α, TNF-β, IL-4, IL-5, IL-7, IL-9, IL-13, CCL11, IL-8, IP-10, MCP-1,
MCP-4, MIP-1α, MIP-1β, RANTES, IL-10, IL-15, IL-22, TGF-β1, IL-1β, IL-6, IL-17A, IL-17F, IL-21,
Granzyme B, Perforin, sCD40L, sCD137

IsoPlexis’ Functional Immune Landscaping Enables Critical Single-Cell Discoveries

Polyfunctionality is considerably increased in samples treated with ACT+NKTR-214 vs ACT+IL-2. NKTR2-14 elicited more polyfunctional cell subsets.

Parisi, G., Saco, J.D., Salazar, F.B. et al. Persistence of adoptively transferred T cells with a kinetically engineered IL-2 receptor agonist. Nat Commun 11, 660 (2020).

Product Specifications