Impedance Assay

How does it work?

  • Measures how easily signal passes the electrode-cell interface
  • Impedance/resistance increases as coverage and attachment increases
  • Can detect:
    • Proliferation
    • Viability
    • Cell-cell coupling strength (barrier function)
    • Migration
    • Cell signaling

Maestro Z Advantage

Intuitive Software

  • Advanced plate mapping
  • Instant analysis and intuitive display
  • Quickly export figures and tables directly to Excel
  • Maestro Z App for remote monitoring
  • FDA 21 CFR Part 11 compliance (GxP)

Automated Acquisition

  • Barcode tracked plates
  • Automatic recording when docked
  • Automated CO2 and temperature control
  • Automatic event tracking records door movements

Interactive Report

  • Lab Notebook
    • Right-click any figure from AxIS Z
    • Export all to single Excel file separated by worksheets
    • Fully editable Excel chart with linked data tables
    • Copy/Paste any plot or data to other software
    • File, platemap, and analysis settings exported for traceability

Applications

Immuno-oncology

Monitor effector-mediated cytolysis of target cancer cell lines

  • Automatically calculate %Cytolysis
  • Determine effective T-cell ratio
  • Compare potency between treatments

Scratch Assay

Monitor cell migration (metastatic potential) and wound healing

MCF-7 (breast cancer line)

Scratch

After 48hr

HCC1806 (Triple-negative breast cancer line)

Scratch

After 48hr

  • Monitor cellular proliferation and invasion into the wound
  • Determine how aggressive a cancer cell line migrates (an indicator of metastatic potential)
  • Determine how effective cells are at repairing damage (non-cancer lines)

Cell Signaling

Monitor rapid receptor-mediated signaling in real time

  • Capture effects that take minutes or hours with the same plate

Cell Proliferation

Unique functional cellular profile

  • Compare growth rates at different plating densities
  • Different cell sources have different growth profiles and maximum impedance

Cytotoxicity

Dynamics of cytotoxicity

  • Instantly calculate % cytolysis
  • Plot any time point
  • Compare kinetics of the compound
    • Calculate Kill Time50%

Virus Study

Track virus-induced cytopathic effects in real-time

Remdesivir prevents cell death from SARS-CoV-2 (COVID-19) virus

  • Cell death was inhibited at 1.38 µM Remdesivir
  • 8.33 µM and 50 µM Remdesivir completely prevented cell death from SARS-CoV-2

Antiviral screening for SARS-CoV-2

  • Each drug prevented cell death from SARS-CoV-2
  • Compounds 3 and 4 were toxic to the cells at highest concentrations
Data courtesy of Drs. Alex Jureka and Chris Basler at Georgia State University

TEER (Trans-Epithelial/endothelial Electrical Resistance) Assay

Measure Barrier Function in Real Time

How it works

  • Maestro Z measures the transepithelial (or transendothelial) electrical resistance, or TEER.
  • A small current is passed between electrodes.
  • TEER measures how much current is blocked by the cellular layer, specifically the “tight junctions” between cells.
  • Continuously monitor for minutes, hours, or even days without disturbing the cellular biology.
Bursting in iPSC-derived ALS Motor Neurons Recorded with Maestro MEA system

Monitor Coverage and TEER Simultaneously

  • Maestro Z can track coverage and TEER simultaneously by measuring at different frequencies.
    • At high frequencies, how many cells determines impedance (coverage)
    • At low frequencies, junction strength has more influence (TEER)
  • Maestro Z continuously monitored cell coverage and barrier function of two lung epithelial cell lines.
    • Both cell lines reach confluence within 24 hours.
    • The Calu-3 cells, which express tight junctions, produce a larger TEER signal, indicating a strong cellular barrier.

Barrier Function

Evaluate barrier function in disease-in-a-dish models

  • Many signaling molecules, such as inflammatory agents and common drugs, can alter barrier permeability.
  • Cytochalasin D inhibits actin polymerization to increase tight junction permeability, as reflected by the rapid decrease in TEER.
  • Ethanol acts more slowly on ZO-1 and Claudin-1, two key tight junction proteins.
  • The rapid disruption of barrier integrity, as well as the mechanistically distinct dynamics, are captured by TEER measurements on the Maestro Z.

Barrier Function

Evaluate barrier function in disease-in-a-dish models

  • Barriers serve critical functions throughout the body, and may be disrupted in disease, such as cystic fibrosis.
  • Calu-3 cells produce tight junctions and express functional cystic fibrosis transmembrane conductance regulator (CFTR).
    • Upon β-adrenergic stimulation, CFTR allows ion transport across the cell membrane to facilitate mucus secretion.
    • Process is disrupted in cystic fibrosis
  • Addition of isoproterenol and forskolin significantly reduced TEER of the Calu-3 cells.
  • CFTR(inh)-172 is an inhibitor of the CFTR.
    • Addition of CFTR(inh)-172 caused an immediate dose-dependent reversal of the isoproterenol and forskolin response.

Product

Consumable

CytoView-Z

The CytoView-Z plates combine robust impedeance data collected with a transparent well bottom for cell visualization and assay multiplexing

*Schematic of well illustraing the recording electrode (blue) and the ground (orange).