SynBBB 3D Blood Brain Barrier Model – Real-time visualization of cellular and barrier functionality
SynVivo’s SynBBB 3D blood brain barrier model recreates the in vivo microenvironment
by emulating a histological slice of brain tissue cells in communication with endothelial cells across the blood brain barrier (BBB). Shear-induced endothelial cell tight junctions are easily achieved in the SynBBB model using physiological fluid flow.
∙ Accurate in vivo hemodynamic shear stress
∙ Real-time visualization of cellular and barrier functionality
∙ Significant reduction in cost and time
∙ Robust and easy to use protocols
∙ Tight junction proteins: Determine the levels of tight junction proteins namely zonula occludens, claudins and occludins which regulate the BBB.
∙ Transporter proteins: Analyze functionality of transporter proteins (e.g. Pgp) in normal and dysfunctional BBB.
∙ Drug permeability: Evaluate real-time permeability of therapeutics and small molecules across the endothelium of the BBB.
∙ Inflammation: Understand the underlying mechanisms of inflammatory responses on the regulation of the BBB.
∙ Cell migration: Visualize and quantify in real-time migration of immune cells across the BBB.
∙ Omic changes: Perform genomic, proteomic and metabolic analysis on normal and dysfunctional BBB.
∙ Neurotoxicity: Analyze toxicity effects of chemical, biological and physical agents on the cells of the BBB.
∙ Neuro-oncology: Investigate effects of the tumor cells on the BBB.
Schematic of the BBB Model. Apical chamber (outer channels) are for culture of vascular (endothelial cells) while basolateral chamber (central chamber) are for culture of brain tissue cells (astrocytes, pericytes, neurons). Porous architecture enables communication between the vascular and tissue cells.