3D Tissue/Organ-on-chip models

SynVivo has developed 3D tissue models that accelerate real-time studies of cellular behavior, drug delivery and drug discovery by providing a morphologically and biologically realistic microenvironment that more accurately depicts in vivo reality. SynVivo models recreate complex in vivo microvasculature including scale, morphology, hemodynamic shear stress and cellular interactions in an in vitro microfluidic chip environment.
These tissue models are morphologically and physiologically realistic with side-by-side architecture enabling real-time visualization.

∙ SynALI – Air Liquid Interface based Lung Model

∙ SynBBB – Blood Brain Barrier Model validated for human or rat species

∙ SynTumor – Various Cancer Models

∙ SynRAM – Inflammation model for rolling adhesion and migration assays

∙ SynTox – Toxicology

Chips designs are based on actual microvascular network imaging or an idealized vasculature network supporting morphologically and physiologically realistic assays across basic and applied life science research.

1. Realistic Microvascular Networks Based 3D Tissue Model: Replicate in vivo physiological, morphological and cellular makeup for cell-cell and cell-drug interaction across the vascular-tissue interface.
2. Idealized Microvascular Network Based 3D Tissue Model: Replicate in vivo fluidic and cellular makeup for cell-cell and cell-drug interaction across the vascular-tissue interface.

SynALI – Air Liquid Interface based Lung Model

SynVivo has developed a novel Air Liquid Interface (ALI) model mimicking the lung architecture. The device comprises of a plastic, disposable and optically clear microfluidic chip functionalized with epithelial cells surrounded by vasculature comprised of endothelial cells.

Unique Features:

∙ Morphologically realistic airway structure and environment

∙ Air Liquid Interface (ALI) across the epithelium and endothelium

∙ in vivo hemodynamic shear stress

∙ Real-time visualization of cellular and barrier functionality including mucus, ciliary beating, immune cell interactions and therapeutic screening

Applications:

∙ Cell morphology

∙ Airway structure

∙ Cell-cell interactions

∙ Functions of the airway

(e.g. mucus transport, ciliary beating, therapeutic-induced improvements)

Kit contents and Description

SynBBB 3D Blood Brain Barrier Model – Real-time visualization of cellular and barrier functionality

SynVivo’s  SynBBB 3D blood brain barrier model recreates the in vivo microenvironment
by emulating a histological slice of brain tissue cells in communication with endothelial cells across the blood brain barrier (BBB). Shear-induced endothelial cell tight junctions are easily achieved in the SynBBB model using physiological fluid flow.

SynBBB is the only in vitro BBB model that allows:

∙ Accurate in vivo hemodynamic shear stress

∙ Real-time visualization of cellular and barrier functionality

∙ Significant reduction in cost and time

∙ Robust and easy to use protocols

Applications:

∙ Tight junction proteins: Determine the levels of tight junction proteins namely zonula occludens, claudins and occludins which regulate the BBB.

∙ Transporter proteins: Analyze functionality of transporter proteins (e.g. Pgp) in normal and dysfunctional BBB.

∙ Drug permeability: Evaluate real-time permeability of therapeutics and small molecules across the endothelium of the BBB.

∙ Inflammation: Understand the underlying mechanisms of  inflammatory responses on the regulation of the BBB.

∙ Cell migration: Visualize and quantify in real-time migration of immune cells across the BBB.

∙ Omic changes: Perform genomic, proteomic and metabolic analysis on normal and dysfunctional BBB.

∙ Neurotoxicity: Analyze toxicity effects of chemical, biological and physical agents on the cells of the BBB.

∙ Neuro-oncology: Investigate effects of the tumor cells on the BBB.

Schematic of the BBB Model. Apical chamber (outer channels) are for culture of vascular (endothelial cells) while basolateral chamber (central chamber) are for culture of brain tissue cells (astrocytes, pericytes, neurons). Porous architecture enables communication between the vascular and tissue cells.

Basic SynBBB Configuration

Analyze experiments using real-time imaging, biochemical and molecular biology methodologies.

TEER Compatible SynBBB Configuration with the SynVivo Cell Impedance Analyzer

Analyze experiments using real-time trans-endothelial electrical resistance (TEER) including real-time imaging, biochemical and molecular biology methodologies.

SynBBB 3D Model Kit Components

SynTumor 3D Cancer Model – Recreating the tumor microenvironment

SynTumor is a 3D tissue model for real-time visualization and quantitative assessment
of cell-cell and cell-drug interactions in a physiologically and morphologically realistic tumor microenvironment.
The system enables (a) circulation in the microvasculature, (b) transport across the vessel walls, and (c) delivery to the tumors.
Starting with scans of vascular networks incorporated with interstitial and tissue/tumor spaces, the SynTumor 3D tissue model creates an in-vitro tumor microenvironment akin to a viable histological slice.

Benefits:

∙ Side by side architecture enables quantitative real time visualization

∙ Physiological leaky vasculature with engineered porous structures

∙ Morphologically realistic in vivo based architecture

∙ Physiologically realistic convective and diffusive transport

∙ Microfluidic platform with ultra-low consumable volumes

Depending on your research needs you can select from the “idealized” or “microvascular” SynTumor 3D Cancer Model configuration.

Create a realistic 3D co-culture with real time monitoring of cell-cell interactions between tumor, stromal, vascular and immune cells.

Idealized Network Configuration

Available with 2µm or 8µm pore configuration.

Microvascular Network Configuration

Available with 2µm or 8µm pore configuration.

SynTumor 3D Model Starter and Assay Kit Components

SynRAM 3D Inflammation Model – Visualize Rolling, Adhesion and Migration in a Single Assay

The SynRAM™ 3D Inflammation Model from SynVivo has been developed to study the
entire inflammation pathway in a realistic and dynamic environment.
By recreating a histological slice of co-cultured tissue and/or tumor cells with a lumen of endothelial cells, the SynVivo platform delivers a physiologically realistic model including flow and shear in a platform and enables real-time tracking of rolling, adhesion and migration processes.

SynRAM 3D inflammation model provides a realistic testing environment including:

∙ Physiological shear stress within a microvascular environment

∙ In vivo like vascular morphology with fully enclosed lumen

∙ Co-culture capability for cell-cell interactions

∙ Quantitative real-time rolling, adhesion, and migration data from a single experiment

SynRAM enables assessment of cellular interactions comprising of rolling, adhesion and migration through multiple cellular layers in one experiment, in real-time, and represents data closely correlated with in vivo results.

Idealized Network

Microvascular Network

SynRAM 3D Model Kit Components

SynTox 3D Toxicology Model – Organ Specific Physiological ResponsesInflammation Model – Visualize Rolling, Adhesion and Migration in a Single Assay

SynTox is the only commercially available 3D toxicology model with real-time optical monitoring and multi-compartment, multi-cellular architecture and low reagent requirements.

Other benefits:

∙ Physiologically realistic morphological, fluidic and 3D cellular conditions

∙ Universal platform with architecture specific of desired organ

∙ Significant reduction in cost and time

∙ Robust and easy to use protocols

∙ Compatible with standard analytical instruments for both on chip and off chip assays including omic methodologies for systems biology and bioinformatic analysis

SynTox 3D Toxicology Model recreates the in vivo micro-environment by emulating a histological slice operating in an in vitro Environment.

SynTox 3D Model Kit Components